Assessment of Molecular Diversity among the Parental Lines of Hybrid Rice (Oryza Sativa.L)
The present study entitled “Assessment of molecular diversity among parental lines of hybrid rice (Oryza sativa.L)” was conducted with 96 parental lines at Division of Genetics, IARI, New Delhi. The objective of the investigation was to study the genetic diversity pattern among parental lines of hybrid rice comprising 31 maintainers and 65 restorers. A total of 50 SSR markers which were shortlisted by IRRI under Generation Challenge Programme for diversity studies were selected and are dispersed across the 12 chromosomes. Of 50 SSR markers, 39 were found to be polymorphic, 1 monomorphic and 10 were not amplified. A total of 173 alleles were amplified by 39 polymorphic markers. Allele no. per locus generated by each marker ranged from 2 to 8 with an average of 4.435 alleles per locus. The value of major allele frequency, gene diversity, heterozygosity and PIC was ranged from 0.30 to 0.89, 0.20 to 0.79, 0.01 to 0.23 and 0.19 to 0.76, respectively. The cluster pattern generated by DARwin software resolved 96 parental lines into three clusters. All three clusters subclustered for maintainer and restorer groups.