Molecular Interaction of Hordeumvulgare Mlaimmune Receptor with MYB6 Transcription Factor through Modeling and Docking Studies
Barley is a major cereal crop from poaceae family grown worldwide and it is one of the first cultivated grains.
Barley has also been used as animal fodder, as a source of fermentable material for beer and certain distilled beverages, and
as a component of various health foods. It is used in soups and stews, and in barley bread of various cultures. Barley grains
are commonly made into malt in a traditional and ancient method of preparation. A number of biotic and abiotic factors
affect the crop yield and quality. Among the biotic factors, barley is susceptible to various diseases, common ones are net
blotch (caused by Drechslerateres), scald (caused by Rhynchosporiumsecalis), leaf rust (caused by Pucciniahordei) and
powdery mildew (caused by Blumeriagraminisf.sp. hordei). Biologists are engaged in controlling the disease and increasing
the immunity against disease. Here, Mildew Locus Allele (MLA6) is triggering immunity against the barley powdery
mildew fungus and MLA6 interact with MYB6 antagonistically acting transcription factor and their cluster provides
immunity against Blumeriagraminis to the barley. Recently, adopting new strategies of bioinformatics for designing of
molecular model based on the concept of basic local alignment and docking approach for protein-protein interactions is used
to generate the 3D model of MLA6and MYB6 through BLASTP and EasyModeller 4.0. Further their generated structures
were docked with E-value -625.66 kCal/mol which provides the insight into the MLA6 – MYB6 molecular interaction which
is the result of MLA triggered immunity in barley. This study can be further used for drug design and plant pathological
Keywords - Barley, Powdery mildew, Blumeriagraminis, MLA6, MYB6, Docking.