Paper Title
Development and Validation of Analytical Method for Assay Determination of Isosulfan Blue by Liquid Chromatography

Abstract
A novel, simple and rapid method has been developed and validated for quantitative assay determination of isosulfan blue in drug substance by Ultra Performance Liquid Chromatography (UPLC). Analytical method was developed and validated as per ICH guidelines. Reverse phase liquid chromatography method is developed and validate on UPLC using C18 (100 x 2.00 mm) 1.9� column. 0.1 % perchloric acid in mixture of 73 volumes of water and 27 volumes of acetonitrile as mobile phase. The ultra violet wavelength selected 220 nm, the column temperature 30�C. Linearity study was carried out for isosulfan blue linearity was calculated from 80% level to 120% with respect to specification level. The correlation coefficient r = 0.999 was proved and showed that the method is linear. Specificity was studied by individual injection of drug substance, related impurities and spiking study as well. It was evident from specificity test that all the related impurities are separated from isosulfan blue peak and method is specific. Isosulfan blue sample solution and mobile phase were found to be stable for at least 3 days. Keywords - Isosulfan blue, UPLC, Assay, Validation, Reverse phase, Solution stability.