Analysis of DNA Damages caused by Santalum album Extract in Hordeum vulgare using DNA Genomic Pattern
Sandalwood is a class of woods from trees in the genus Santalum. Sandalwood is the second most expensive wood in the world and used as flavoring agent in cosmetics, but it may be mutagenic for the stability of genome and the normal growth of the plants. An experiment was conducted to determine the effect of santalum album extract in Hordeum vulgare. The steadiness of genome and growth of Hordeum vulgare plant affected by DNA damage had been observed when Hordeum vulgare treated with different concentration of santalum album extract (100, 200, 300, 400, & 500µl/mL) through a variety of mechanisms and one of the method had been to used to isolate the DNA is C-TAB method. This study is arrived at evaluating DNA damages caused by Santalum album extract in Hordeum vulgare. The major difference had been observed by utilizing the experiment that had been performed to analyze the DNA damage by Agarose gel electrophoresis. In this experiment, different concentration of Santalum album was used to analyze the DNA damages in Hordeum vulgare, and when we increased the concentration percentage from low to high dose, the intensity and brightness of the bands shown in each well decreased and vice versa, which implies that DNA had been thoroughly degraded. The experimental data were analyzed that Santalum album had significant effect on genomic DNA pattern.
Keywords - DNA degraded, Mutagenic, C-TAB method, Electrophoresis, Santalum album extract, Hordeum vulgare plant.