Isolation and Characterization of Probiotic Bacteria from Lutjanuscampechanus
Probiotics are live bacteria or yeast cells that are characterized by having beneficial effects, commonly to the digestive system. Additionally, they balance the good and bad bacteria in the stomach in order to optimize bodily functions. Bacteria from the gut cells of the ecologically available fish species, Lutjanuscampechanus were isolated to check their probiotic activity. The gut sample was inoculated in NB broth resulting in the isolation of two colonies and various microbiological analyses were carried out on the colonies. The antibacterial activity of these organisms was tested against fish gut pathogens and an antibiogram was carried out to understand the drug resistance pattern of these organisms. Additionally, the pH and bile tolerance of the colonies were tested for optimum values.
Keywords - Probiotics, Lutjanus Campechanus, Antibacterial Assay, Antibiogram, pH Tolerance, Bile Tolerance