In Vitro Anticancer Activity of Lectin Isolated from Gel of Aloe Barbadensis (Miller) against Hela and SW480 Human Cancer Cell Lines
Lectin is a glycoprotein also called haemagglutinating protein which has ability to agglutinate all types of human blood. This paper presents the anticancer activity of lectin isolated from gel of Aloe barbadensis (Miller). The glycoprotein from the 0.9% of normal saline extracts of Aloe barbadensis leaves was purified by dialysis with 50% of ammonium sulfate. Protein concentration was determined by Lowry’s method. The haemagglutination assay was done to confirm the presence of lectin in crude extract. Sugar specificity assay was carried out in order to determine the effect of different carbohydrate on haemagglutination and thus find specific carbohydrate for Aloe lectin. The dialyzed sample allowed for SDS-PAGE to determine the molecular weight (Mr). The anticancer activity has assessed by SRB assay after 48hours.
Keywords - Anticancer Activity, Aloe barbadensis, Haemagglutination Assay, Lectin, SRB Assay