Paper Title
Human Papilloma Virus is a Major Risk Factor in Breast Cancer Development and Viral DNA Detection in Plasma is a Prognostic Biomarker in Patients from West Bengal, India.

Abstract
Breast Cancer is the most common cancer among the worldwide women. It requires molecular alterations along with human papilloma virus (HPV) infection for development and progression. In our study, we have analysed HPV association in Breast cancer in patients from West Bengal, India, attending at Chittaranjan National Cancer Institute, Kolkata. Samples of tumor tissues, blood plasma andadjacent normal tissues were examined for HPV infection, among which 68% of the samples were HPV+ve of which 68% of the samples were HPV16 +ve followed by HPV 18 (28%) and HPV 33 (4%). Both tumor tissue and plasma infections were evident in 38% of the samples. Human Papilloma viral DNA was found in 82% of the plasma samples alone and showed statistically significant association with disease free survival. Immunohistochemical analysis of E6 and E7 viral oncoprotein expressions were found to be high/medium in 70% and 50% of the samples respectively. Statistical analysis showed significant association with poor patient survivality withhigh/medium E6 expression than E7 oncoprotein expression. Keywords - Breast Cancer, Human Papilloma Virus, Plasma Viral DNA Detection, Prognostic Biomarker.