Paper Title
PHYLOGENETIC ANALYSIS OF THE MICROBES ISOLATED FROM ORAL CAVITY OF INDIVIDUALS CONSUMING INTOXICATING SUBSTANCES

Abstract
Abstract - Food patterns and oral cleanliness are significant variables in the physiochemical environment of the mouth cavity. Intoxicating substances such as betel nut ('Tamul,' alcohol, smoking, and chewing tobacco can all have a significant impact on the oral ecology, including commensal microorganisms. As a result, a comparison of microorganisms in the oral cavity between those who consume intoxicating substances and those who do not could indicate the influence of these substances. Microbes were isolated by culturing on Nutrient agar and identified by phylogenetic analysis of their 16S rRNA gene sequences from oral swabs taken from intoxicating substance consumers and non-consumers in Assam, India. The effects of intoxicating substances on the occurrence of microorganisms and health problems were assessed using binary logistic regression. Pseudomonas aeruginosa, Serratia marcescens, Rhodococcus antrifimi, Paenibacillus dendritiformis, Bacillus cereus, Staphylococcus carnosus, Klebsiella michiganensis, and Pseudomonas cedrina were the most common pathogens and opportunistic pathogens found in the oral cavity of consumers and oral cancer patients. Enterobacter hormaechei was found in the oral cavity of cancer patients but not in the oral cavity of other individuals. Pseudomonas spp. were found to be widespread. On exposure to various intoxicating substances, the chance of occurrence of these organisms was found to be between 0.01 and 2.963 odds, and the risk of health issues was found to be between 0.088 and 10.148 odds. The risk of various health issues when exposed to bacteria ranged between 0.108 to 2.306 odds. Chewing tobacco increased the risk of oral cancer (10.148 odds). Prolonged exposure to intoxicated substances creates an ideal habitat for infections and opportunistic pathogens to colonize in the oral cavity of those who consume intoxicating substances.