Paper Title
Bioprospecting of Secondary Metabolites and Impact of Biogenic Zno Nanoparticles in in Vitro Callus Induction of Medicinally Important Curcuma Aeruginosa Roxb.

Abstract
Curcuma aeruginosaRoxb. is a stemless, aromatic rhizomatous plant, with extensive biological activity such as anticancer, antioxidant, antimicrobial, anti-dengue, anthelmintic, anti-inflammatory, anti-androgenic, immune-stimulating, anti-nociceptive, antipyretic activity, and uterine relaxant effect. The authentic reports on the phytochemical aspects of this plant in Kerala is very few with absence of studies in the establishment of callus cultures. Present study reports on the successful optimization of in vitro callus induction from young rhizome of C.aeruginosa and the impact of elicitation employing phyto-derived ZnO nanoparticles. The bioprospecting was performed using standard qualitative tests, total phenolic and flavonoid content quantification, HPTLC and GCMS analysis. The results reveals the presence of phenolic, flavonoid, mono/triterpenes, terpenoids, anthocyanidinsetc. Further the plant hormonal combination of 2,4-D and BAP in MS medium was standardized for callus induction of this valuable plant. Phyto-derived ZnO nanoparticles was synthesized employing the aqueous extract of the rhizome of C.aeruginosa and they are characterized using XRD, FTIR, SEM analysis. The incorporation of this nano-elicitor to the medium with and without plant growth regulators showed guaranteed effects in the callus culture.