Paper Title
ISOLATION AND IDENTIFICATION OF SOIL MICROORGANISMS BACILLUS SUBTILIS, PSEUDOMONAS FLUORESCENS, AND LACTOBACILLUS PLANTARUM FROM BANSUR AREA OF RAJASTHAN, INDIA – A CASE STUDY

Abstract
Isolation and identification of Bacillus subtilis, Pseudomonas fluorescens, and Lactobacillus plantarum bacteria from soil in the Bansur region of Rajasthan, India, are the main objectives of this work. Thirty (30) bacterial isolates were extracted from the soil. Three (03) of these isolates were identified as Bacillus subtilis. It was discovered that five (05) were Pseudomonas fluorescens and four of them were Lactobacillus plantarum. Use morphological and biochemical tests to further characterize them. Colonies of Bacillus subtilis strains were medium in size, round, opaque, and white. There were motile, urease, catalase, nitrate reduction, citrate utilization, vogues proskauer, TSI positive, oxidase negative, and gram-positive rods. The colonies of Pseudomonas fluorescens strains were irregular, tiny, smooth, opaque, and pale green. Indole, methyl red, vogues proskauer negative reaction, motile, urease, oxidase, catalase, citrate utilization, pigment producing positive, and gram negative rods were all present. The strains of Lactobacillus plantarum displayed tiny, spherical, smooth, translucent, and whitish colonies. Gram-positive rods, methyl red, glucose, ribose, sorbitol, xylose, fructose, nitrate reduction, and negative reactions for catalase, oxidase, vogues proskauer, indole galactose, and maltose were seen. These bacteria can be used to decompose food waste. Keywords - Soil Microorganism, Morphology, Biochemical Analysis, Isolation & Identification