Paper Title
Plant Abiotic Stress Challenges from the Changing Environment: How to Develop Plants Capable of Mitigating Climate Change
Abstract
Climate change is a multifaceted phenomenon with a wide range of impacts on the environment. Currently, with
the competing uses of land and the growing world population, we are challenged to produce more in less area with
diminishing resources, confronted with climate change and the unpredictable local microclimate adversely affecting crop
productivity. Biotic and abiotic stress is a result of climate change. Abiotic stresses will remain a challenge to the natural
environment and agriculture. The challenges before us in plant biology and crop improvement are to integrate the systems
level information on abiotic stress response pathways, identify stress protective networks, and engineer environmentally
stable crops that yield more. Plants evolve defense mechanisms to withstand these stresses, e.g. antioxidants and antioxidant
enzymes. In the present study, two different antioxidant enzymes namely copper-zinc superoxide dismutase derived from
Potentilla astrisanguinea (Cu-Zn/SOD) and ascorbate peroxidase (APX) from Rheum austral both of which are high altitude
cold niche area plants of Western Himalaya were cloned and simultaneously over-expressed in Arabidopsisthaliana to
alleviate salt stress. It was found that the transgenic plants over-expressing both the genes were more tolerant to salt stress
than either of the single gene expressing transgenic plants during growth and development. Further, transcriptomic analysis
showed that most of the genes related to secondary metabolite production and phytohormones were over expressed in
transgenic lines under stress conditions. Thus, genetically engineered plants or biotech crops can contribute significantly
both to sustainability and for the mitigation of the arduous challenges associated with possible climate change and global
warming.
Keywords - Arabidopsis thaliana, Salinity, RNA sequencing, Phytohormones, Secondary metabolites, Climate Change