Paper Title
VI CAPSULAR POLYSACCHARIDE CONJUGATE WITH CARRIER PROTEIN METHODS AND CHARACTERIZATION OF VI CAPSULAR POLYSACCHARIDE CONJUGATE

Abstract
In this study it was demonstrated that Vi polysaccharide conjugates with carrier protein of various methods. The Vi polysaccharide conjugates with carrier protein prepared in two methods: first method was Vi polysaccharide directly bind with carrier protein in presence of ethylene carbodiimide and second method was carrier protein binding with adipic acid dihydrazide (ADH) spacer molecules in presence of ethylene carbodiimdide and further it bind to Vi polysaccharide. The Vi polysaccharide- carrier protein conjugate size was increased and purified with sepharose CL 4B matrix and it eliminates the un-conjugate molecules of Free Vi polysaccharide and carrier protein. The Vi polysaccharide- carrier protein conjugates was tested as per the specification gives in Technical report serious and result were complied. Keywords - Salmonella typhi, Vi capsular polysaccharide, Adipicdihydrazide (ADH), EDC (ethylene carbodiimide) and Sepharose CL 4B.