Paper Title
Evaluation of Nutrititive Potential of Bioactive Peptides Isolated from The Spotted Snakehead, Channa Punctata

Abstract
The spotted snakehead (Channapunctata; Family: Channidae), is an indigenous, obligate air breathing, freshwater fish. Proximate analysis reveals that dried fish muscle contains high amount of protein (64.42±0.51%), moderate amount of carbohydrate (11.92±0.42%), reducing sugar (15.13±0.68%) and low quantity of lipid (2.97±0.29%) and ash (4.29±0.55%). Fatty acid analysis reveals presence of high amount of ω-3 fatty acids: eicosapentaenoic acid (9.44±0.07) and docosahexaenoic acid (14.66±0.31) which has high prophylactic values. Amino acid analysis shows presence of good amount of essential amino acids. Functional properties of muscle protein hydrolysates using pepsin and Alcalase 2.4L were evaluated. The rate of degree of hydrolysis was found to reach maximum 80% in case of 240 minutes of hydrolysis with Alcalase 2.4L. Functional properties of muscle protein hydrolysates using pepsin and Alcalase 2.4L were evaluated. Antioxidative assay with ultrafiltrate (<3kDa) of 240 minutes pepsin hydrolysate (PepHyd240) has maximum ability to scavenge the free radicals. Alcalasehydrolysates of 240 minutes (AlcHyd240) peptide fractions (<3kDa) having ACE inhibitory activity with lowestIC50 value were sequenced by MALDI-TOF and matched with previously recognised ACE inhibitory peptides. Utilisation of such low molecular weight peptides can help to address the health issues by using them in various food formulations. Keywords - Channapunctata, Protein Hydrolysates, Bioactive Peptides, Antioxidant Activity, Antihypertensive Activity, MALDI-TOF.