Paper Title
Development and Validation of Analytical Method for Estimation of Desvenlafaxine by RP-HPLC

Abstract
A new, simple, precise, accurate and reproducible reverse phase high performance liquid chromatography (RP-HPLC) method is developed for Desvenlafaxine (DV). The estimation of DV was successfully achieved on Hi-Q-Sil C-18 (150 mm × 4.6 mm) analytical column, Acetonitrile: phosphate buffer pH 4.6 (30: 70 v/v) mobile phase at a flow rate of 1.0ml/min and eluate was monitored at 230nm, with a retention time of 6.637 minutes for DV. The methodwas validated and the response was found to be linear in the drug concentration range of 20 to120 μg/ml for DV. The values of the correlation coefficient were found to 0.999for DV. The Limit of Detection (LOD) and Limit of Quantification (LOQ) for DV were found to be 1.8147 µg/ml and 5.4992 µg/ml respectively. The % recovery was found to be 99.8% indicating that the proposed method is highly accurate. The specificity of the method shows good correlation between retention times of standard with the sample so, the method specifically determines the analyte in the sample without interference from excipients of tablet dosage forms. The method was extensively validated according to International Council for Harmonisation (ICH) guidelines