Paper Title
Green Synthesis of Silver Nanoparticle & Determination of its Antimicrobial Activity

Abstract
An important development in the world of nanotechnology is the ecologically friendly synthesis of nanoparticles. Due to its ease of use and environmental friendliness, plant-mediated biological production of nanoparticles has become more significant in recent years.This study reports the utilization of apple extract for the synthesis of silver nanoparticles to determine their antimicrobial activity. The biosynthesized AgNPs were confirmed by using different characterization techniques such as XRD, FTIR, UV-Vis spectroscopy, and SEM. The antimicrobial activity was also carried out against the P. aeruginosa, E. coli, S. aureus and B. cereus bacteria and fungus isolates of C. albicans, P. chrysogenum, A. nigerand T.viridiae. During the antimicrobial activity, the bacteria and fungus showed great inhibition to the AgNPs at a concentration of 80 µl. The AgNPs have eliminated the bacteria at different concentrations with higher antibacterial activity for P. aeruginosa>E. coli> S. aureus>B. cereuswhereas all the fungal strains showed a ZOI of 8 mm when treated with the apple extract synthesized AgNPs. The data strongly suggest that the AgNPssynthesised with apple extract have a potential antimicrobial activity.